Reaction: L-aspartate + NAD+ = 2-iminosuccinate + NADH + H+
Other name(s): bhcD (gene name)
Systematic name: L-aspartate:NAD+ oxidoreductase (iminosuccinate-forming)
Comments: The enzyme, characterized from the bacterium Paracoccus denitrificans, participates in the the β-hydroxyaspartate cycle of glyoxylate assimilation. The substrate, 2-iminosuccinate, is very unstable, and spontaneously decays into free ammonia and oxaloacetate in the absence of the enzyme. cf. EC 1.4.1.21, aspartate dehydrogenase, which acts in the opposite direction, producing 2-iminosuccinate that transforms into ammonia and oxaloacetate.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Schada von Borzyskowski, L., Severi, F., Kruger, K., Hermann, L., Gilardet, A., Sippel, F., Pommerenke, B., Claus, P., Cortina, N.S., Glatter, T., Zauner, S., Zarzycki, J., Fuchs, B.M., Bremer, E., Maier, U.G., Amann, R.I. and Erb, T.J. Marine Proteobacteria metabolize glycolate via the β-hydroxyaspartate cycle. Nature 575 (2019) 500-504. [PMID: 31723261]