IUBMB Enzyme Nomenclature


Accepted name: nicotine dehydrogenase

Reaction: (S)-nicotine + 2 ferricytochrome c = N-methylmyosmine + 2 ferrocytochrome c + 2 H+

Glossary: (S)-nicotine = 3-[(S)-1-methylpyrrolidin-2-yl]pyridine
N-methylmyosamine = 3-(1-methyl-4,5-dihydro-1H-pyrrol-2-yl)pyridine

Other name(s): nicA2 (gene name)

Systematic name: (S)-nicotine:cytochrome c oxidoreductase (N-methylmimosine-forming)

Comments: The enzyme, characterized from the bacterium Pseudomonas putida S16, contains an FAD cofactor and belongs to the flavin-containing amine oxidase family. The enzyme from this bacterium is specific for the c-type cytochrome CycN. The product undergoes spontaneous hydrolysis to form pseudooxynicotine.

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number:


1. Tang, H., Wang, L., Wang, W., Yu, H., Zhang, K., Yao, Y. and Xu, P. Systematic unraveling of the unsolved pathway of nicotine degradation in Pseudomonas. PLoS Genet. 9 (2013) e1003923. [PMID: 24204321]

2. Dulchavsky, M., Clark, C.T., Bardwell, J.CA. and Stull, F. A cytochrome c is the natural electron acceptor for nicotine oxidoreductase. Nat. Chem. Biol. 17 (2021) 344-350. [PMID: 33432238]

[EC created 2022]

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