IUBMB Enzyme Nomenclature


Accepted name: pseudooxynicotine dehydrogenase

Reaction: pseudooxynicotine + H2O + 2 ferricytochrome c = 4-oxo-4-(pyridin-3-yl)butanal + methylamine + 2 ferrocytochrome c + 2 H+

Glossary: pseudooxynicotine = 4-(methylamino)-1-(pyridin-3-yl)butan-1-one

Other name(s): pnaO (gene name)

Systematic name: 4-(methylamino)-1-(pyridin-3-yl)butan-1-one:c-type cytochrome oxidoreductase (methylamine releasing)

Comments: Contains one non-covalently bound FAD molecule per dimer. This enzyme, characterized from the soil bacteria Pseudomonas sp. HZN6 and Pseudomonas putida S16, is involved the nicotine degradation.

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number:


1. Qiu, J., Ma, Y., Wen, Y., Chen, L., Wu, L. and Liu, W. Functional identification of two novel genes from Pseudomonas sp. strain HZN6 involved in the catabolism of nicotine. Appl. Environ. Microbiol. 78 (2012) 2154-2160. [PMID: 22267672]

2. Choudhary, V., Wu, K., Zhang, Z., Dulchavsky, M., Barkman, T., Bardwell, J.CA. and Stull, F. The enzyme pseudooxynicotine amine oxidase from Pseudomonas putida S16 is not an oxidase, but a dehydrogenase. J. Biol. Chem. (2022) 102251. [PMID: 35835223]

[EC created 2012 as EC, transferred 2022 to EC]

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