IUBMB Enzyme Nomenclature


Accepted name: pre-mycofactocin synthase

Reaction: 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + O2 + H2O = 5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidine-2,3-dione + NH3 + H2O2 (overall reaction)
(1a) 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + O2 = 5-[(4-hydroxyphenyl)methyl]-3-imino-4,4-dimethylpyrrolidin-2-one + H2O2
(1b) 5-[(4-hydroxyphenyl)methyl]-3-imino-4,4-dimethylpyrrolidin-2-one + H2O = 5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidine-2,3-dione + NH3 (spontaneous)

For diagram of reaction click here

Glossary: 5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidine-2,3-dione = pre-mycofactocinone = PMFT

Other name(s): mftD (gene name)

Systematic name: 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethyl-2-pyrrolidin-2-one:oxygen oxidoreductase

Comments: A flavoprotein (FMN). The enzyme participates in the biosynthesis of the enzyme cofactor mycofactocin. The enzyme uses oxygen as an electron source to oxidize a C-N bond, followed by spontaneous exchange with water to form an α-keto moiety on the resulting molecule.

Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:


1. Ayikpoe, R.S. and Latham, J.A. MftD catalyzes the formation of a biologically active redox center in the biosynthesis of the ribosomally synthesized and post-translationally modified redox cofactor mycofactocin. J. Am. Chem. Soc. 141 (2019) 13582-13591. [PMID: 31381312]

[EC created 2020]

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