IUBMB Enzyme Nomenclature


Accepted name: sarcosine oxidase (formaldehyde-forming)

Reaction: sarcosine + H2O + O2 = glycine + formaldehyde + H2O2

Other name(s): MSOX; monomeric sarcosine oxidase; sarcosine oxidase (ambiguous)

Systematic name: sarcosine:oxygen oxidoreductase (demethylating)

Comments: The enzyme, reported from bacteria and fungi, catalyses the oxidative demethylation of sarcosine. It contains a FAD cofactor bound to an L-cysteine residue. cf. EC, sarcosine oxidase (5,10-methylenetetrahydrofolate-forming).

Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 9029-22-5


1. Mori, N., Sano, M., Tani, Y. and Yamada, H. Purification and propertie of sarcosine oxidase from Cylindrocarpon didymum M-1. Agric. Biol. Chem. 44 (1980) 1391-1397.

2. Nishiya, Y. and Imanaka, T. Cloning and sequencing of the sarcosine oxidase gene from Arthrobacter sp. TE1826. J. Ferment. Bioeng. 75 (1993) 239-244.

3. Nishiya, Y. and Imanaka, T. Alteration of substrate specificity and optimum pH of sarcosine oxidase by random and site-directed mutagenesis. Appl. Environ. Microbiol. 60 (1994) 4213-4215. [PMID: 16349451]

4. Trickey, P., Wagner, M.A., Jorns, M.S. and Mathews, F.S. Monomeric sarcosine oxidase: structure of a covalently flavinylated amine oxidizing enzyme. Structure 7 (1999) 331-345. [PMID: 10368302]

5. Wagner, M.A., Trickey, P., Chen, Z.W., Mathews, F.S. and Jorns, M.S. Monomeric sarcosine oxidase: 1. Flavin reactivity and active site binding determinants. Biochemistry 39 (2000) 8813-8824. [PMID: 10913292]

6. Zhao, G., Bruckner, R.C. and Jorns, M.S. Identification of the oxygen activation site in monomeric sarcosine oxidase: role of Lys265 in catalysis. Biochemistry 47 (2008) 9124-9135. [PMID: 18693755]

7. Jorns, M.S., Chen, Z.W. and Mathews, F.S. Structural characterization of mutations at the oxygen activation site in monomeric sarcosine oxidase. Biochemistry 49 (2010) 3631-3639. [PMID: 20353187]

8. Bucci, A., Yu, T.Q., Vanden-Eijnden, E. and Abrams, C.F. Kinetics of O2 entry and exit in monomeric sarcosine oxidase via Markovian milestoning molecular dynamics. J Chem Theory Comput 12 (2016) 2964-2972. [PMID: 27168219]

[EC created 1961, modified 2022]

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