IUBMB Enzyme Nomenclature


Accepted name: glyphosate oxidoreductase

Reaction: 2 glyphosate + O2 = 2 aminomethylphosphonate + 2 glyoxylate

Glossary: glyphosate = N-(phosphonomethyl)glycine

Other name(s): gox (gene name)

Systematic name: glyphosate oxidoreductase (aminomethylphosphonate-forming)

Comments: The enzyme, characterized from the bacterium Ochrobactrum sp. G-1, contains an FAD cofactor. The catalytic cycle starts with a reduction of the FAD cofactor by one molecule of glyphosate, yielding reduced FAD and a Schiff base of aminomethylphosphonate with glyoxylate that is hydrolysed to the single components. The reduced FAD is reoxidized by oxygen, generating water and an oxygenated flavin intermediate, which catalyses the oxygenation of a second molecule of glyphosate, forming the second pair of aminomethylphosphonate and glyoxylate.

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number:


1. Barry, G. F. and Kishore. G. M. Glyphosate tolerant plants. US patent US5463175, (1995).

2. Sviridov, A.V., Shushkova, T.V., Zelenkova, N.F., Vinokurova, N.G., Morgunov, I.G., Ermakova, I.T. and Leontievsky, A.A. Distribution of glyphosate and methylphosphonate catabolism systems in soil bacteria Ochrobactrum anthropi and Achromobacter sp. Appl. Microbiol. Biotechnol. 93 (2012) 787-796. [PMID: 21789492]

[EC created 2016]

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