IUBMB Enzyme Nomenclature


Accepted name: NADH oxidase (H2O-forming)

Reaction: 2 NADH + 2 H+ + O2 = 2 NAD+ + 2 H2O

Other name(s): H2O-forming NADH oxidase; Nox-2

Systematic name: NADH:oxygen oxidoreductase (H2O-forming)

Comments: A flavoprotein (FAD). The bacterium Streptococcus mutans contains two distinct NADH oxidases, a H2O-forming enzyme and a H2O2-forming enzyme (cf. EC, NADH oxidase (H2O2-forming)) [1].

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number:


1. Schmidt, H.L., Stocklein, W., Danzer, J., Kirch, P. and Limbach, B. Isolation and properties of an H2O-forming NADH oxidase from Streptococcus faecalis. Eur. J. Biochem. 156 (1986) 149-155. [PMID: 3082630]

2. Higuchi, M., Shimada, M., Yamamoto, Y., Hayashi, T., Koga, T. and Kamio, Y. Identification of two distinct NADH oxidases corresponding to H2O2-forming oxidase and H2O-forming oxidase induced in Streptococcus mutans. J. Gen. Microbiol. 139 (1993) 2343-2351. [PMID: 8254304]

3. Matsumoto, J., Higuchi, M., Shimada, M., Yamamoto, Y. and Kamio, Y. Molecular cloning and sequence analysis of the gene encoding the H2O-forming NADH oxidase from Streptococcus mutans. Biosci. Biotechnol. Biochem. 60 (1996) 39-43. [PMID: 8824824]

4. Kawasaki, S., Ishikura, J., Chiba, D., Nishino, T. and Niimura, Y. Purification and characterization of an H2O-forming NADH oxidase from Clostridium aminovalericum: existence of an oxygen-detoxifying enzyme in an obligate anaerobic bacteria. Arch. Microbiol. 181 (2004) 324-330. [PMID: 15014929]

5. Zhang, Y.W., Tiwari, M.K., Gao, H., Dhiman, S.S., Jeya, M. and Lee, J.K. Cloning and characterization of a thermostable H2O-forming NADH oxidase from Lactobacillus rhamnosus. Enzyme Microb. Technol. 50 (2012) 255-262. [PMID: 22418266]

[EC created 2013]

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