IUBMB Enzyme Nomenclature


Accepted name: protein dithiol oxidoreductase (disulfide-forming)

Reaction: a [DsbA protein] carrying a disulfide bond + a [protein] with reduced L-cysteine residues = a [DsbA protein] with reduced L-cysteine residues + a [protein] carrying a disulfide bond

Other name(s): dsbA (gene name)

Systematic name: protein dithiol:[DsbA protein] oxidoreductase (protein disulfide-forming)

Comments: DsbA is a periplasmic thiol:disulfide oxidoreductase found in Gram-negative bacteria that promotes protein disulfide bond formation. DsbA contains a redox active disulfide bond that is catalytically transferred via disulfide exchange to a diverse range of newly translocated protein substrates. The protein is restored to the oxidized state by EC, protein dithiol:quinone oxidoreductase DsbB.

Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:


1. Bardwell, J.C., McGovern, K. and Beckwith, J. Identification of a protein required for disulfide bond formation in vivo. Cell 67 (1991) 581-589. [PMID: 1934062]

2. Akiyama, Y., Kamitani, S., Kusukawa, N. and Ito, K. In vitro catalysis of oxidative folding of disulfide-bonded proteins by the Escherichia coli dsbA (ppfA) gene product. J. Biol. Chem 267 (1992) 22440-22445. [PMID: 1429594]

3. Zapun, A., Bardwell, J.C. and Creighton, T.E. The reactive and destabilizing disulfide bond of DsbA, a protein required for protein disulfide bond formation in vivo. Biochemistry 32 (1993) 5083-5092. [PMID: 8494885]

4. Bader, M., Muse, W., Zander, T. and Bardwell, J. Reconstitution of a protein disulfide catalytic system. J. Biol. Chem 273 (1998) 10302-10307. [PMID: 9553083]

5. Guddat, L.W., Bardwell, J.C. and Martin, J.L. Crystal structures of reduced and oxidized DsbA: investigation of domain motion and thiolate stabilization. Structure 6 (1998) 757-767. [PMID: 9655827]

6. Kadokura, H., Tian, H., Zander, T., Bardwell, J.C. and Beckwith, J. Snapshots of DsbA in action: detection of proteins in the process of oxidative folding. Science 303 (2004) 534-537. [PMID: 14739460]

[EC created 2019]

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