Reaction: an aliphatic sulfonate + 2 H2O + 4 oxidized ferredoxin = a carboxylate + sulfite + 4 reduced ferredoxin + 4 H+ (overall reaction)
(1a) an aliphatic sulfonate + H2O + 2 oxidized ferredoxin = an aldehyde + sulfite + 2 reduced ferredoxin + 2 H+
(1b) an aldehyde + H2O + 2 oxidized ferredoxin = a carboxylate + 2 reduced ferredoxin + 2 H+
Other name(s): WOR5; PF1479 (locus name); PF1480 (locus name)
Systematic name: aliphatic sulfonate:ferredoxin oxidoreductase (sulfite-releasing)
Comments: An oxygen-sensitive enzyme that contains tungsten-molybdopterin and iron-sulfur clusters, isolated the hyperthermophilic archaeon Pyrococcus furiosus. In that organism the enzyme is heterodimeric, comprising a catalytic subunit that contains the active site tungsto-bispyranopterin cofactor and a [4Fe-4S]cluster and an electron-transfer subunit that contains four additional [4Fe-4S] clusters. The activity involves two steps: an oxidative desulfonation reaction, followed by the activation of a second water molecule and oxidation of the resulting aldehyde (cf. EC 1.2.7.5, aldehyde ferredoxin oxidoreductase).
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Mathew, L.G., Haja, D.K., Pritchett, C., McCormick, W., Zeineddine, R., Fontenot, L.S., Rivera, M.E., Glushka, J., Adams, M.WW. and Lanzilotta, W.N. An unprecedented function for a tungsten-containing oxidoreductase. J. Biol. Inorg. Chem. 27 (2022) 747-758. [PMID: 36269456]