Reaction: 2 ferricytochrome c + selenite + H2O = 2 ferrocytochrome c + selenate
Other name(s): serA (gene name); serB (gene name); serC (gene name); selenite:reduced acceptor oxidoreductase; selenate reductase
Systematic name: ferricytochrome c:selenite oxidoreductase
Comments: The periplasmic enzyme from the bacterium Thauera selenatis is a complex comprising three heterologous subunits (α, β and γ) that contains a guanylyl molybdenum cofactor, as well as multiple iron-sulfur clusters and heme b. The enzyme reduces selenate to selenite using electrons provided by a diheme cytochrome c4, which receives the electrons from the membrane quinone pool via EC 7.1.1.8, quinol—cytochrome-c reductase. Nitrate, nitrite, chlorate and sulfate are not substrates.
Links to other databases: BRENDA, EXPASY, Gene, KEGG, MetaCyc, CAS registry number: 146359-71-9
References:
1. Macy, J.M., Rech, S., Auling, G., Dorsch, M., Stackebrandt, E. and Sly, L.I. Thauera selenatis gen. nov., sp. nov., a member of the β subclass of Proteobacteria with a novel type of anaerobic respiration. Int. J. Syst. Bacteriol. 43 (1993) 135-142. [PMID: 8427805]
2. Schröder, I., Rech, S., Krafft, T. and Macy, J.M. Purification and characterization of the selenate reductase from Thauera selenatis. J. Biol. Chem. 272 (1997) 23765-23768. [PMID: 9295321]
3. Stolz, J.F. and Oremland, R.S. Bacterial respiration of arsenic and selenium. FEMS Microbiol. Rev. 23 (1999) 615-627. [PMID: 10525169]
4. Krafft, T., Bowen, A., Theis, F. and Macy, J.M. Cloning and sequencing of the genes encoding the periplasmic-cytochrome B-containing selenate reductase of Thauera selenatis. DNA 10 (2000) 365-377. [PMID: 10826693]
5. Maher, M.J. and Macy, J.M. Crystallization and preliminary X-ray analysis of the selenate reductase from Thauera selenatis. Acta Crystallogr. D Biol. Crystallogr. 58 (2002) 706-708. [PMID: 11914503]
6. Maher, M.J., Santini, J., Pickering, I.J., Prince, R.C., Macy, J.M. and George, G.N. X-ray absorption spectroscopy of selenate reductase. Inorg. Chem. 43 (2004) 402-404. [PMID: 14730999]
7. Lowe, E.C., Bydder, S., Hartshorne, R.S., Tape, H.L., Dridge, E.J., Debieux, C.M., Paszkiewicz, K., Singleton, I., Lewis, R.J., Santini, J.M., Richardson, D.J. and Butler, C.S. Quinol-cytochrome c oxidoreductase and cytochrome c4 mediate electron transfer during selenate respiration in Thauera selenatis. J. Biol. Chem. 285 (2010) 18433-18442. [PMID: 20388716]