IUBMB Enzyme Nomenclature


Accepted name: cobalt-precorrin-4 methyltransferase

Reaction: S-adenosyl-L-methionine + cobalt-precorrin-4 = S-adenosyl-L-homocysteine + cobalt-precorrin-5A

For diagram of reaction click here.

Other name(s): CbiF

Systematic name: S-adenosyl-L-methionine:cobalt-precorrin-4 11-methyltransferase

Comments: This enzyme, which participates in the anaerobic (early cobalt insertion) cobalamin biosynthesis pathway, catalyses the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5A. See EC, precorrin-4 C11-methyltransferase, for the equivalent enzyme that participates in the aerobic cobalamin biosynthesis pathway.

Comments: Part of the anaerobic route to adenosylcobalamin.

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number:


1. Raux, E., Schubert, H.L., Woodcock, S.C., Wilson, K.S. and Warren, M.J. Cobalamin (vitamin B12) biosynthesis--cloning, expression and crystallisation of the Bacillus megaterium S-adenosyl-L-methionine-dependent cobalt-precorrin-4 transmethylase CbiF. Eur. J. Biochem. 254 (1998) 341-346. [PMID: 9660189]

2. Schubert, H.L., Wilson, K.S., Raux, E., Woodcock, S.C. and Warren, M.J. The X-ray structure of a cobalamin biosynthetic enzyme, cobalt-precorrin-4 methyltransferase. Nat. Struct. Biol. 5 (1998) 585-592. [PMID: 9665173]

3. Kajiwara, Y., Santander, P.J., Roessner, C.A., Perez, L.M. and Scott, A.I. Genetically engineered synthesis and structural characterization of cobalt-precorrin 5A and -5B, two new intermediates on the anaerobic pathway to vitamin B12: definition of the roles of the CbiF and CbiG enzymes. J. Am. Chem. Soc. 128 (2006) 9971-9978. [PMID: 16866557]

[EC created 2013]

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