IUBMB Enzyme Nomenclature


Accepted name: tRNA (guanosine18-2'-O)-methyltransferase

Reaction: S-adenosyl-L-methionine + guanosine18 in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA

Other name(s): tRNA (Gm18) 2'-O-methyltransferase; tRNA (Gm18) methyltransferase; TrmH; SpoU

Systematic name: S-adenosyl-L-methionine:tRNA (guanosine18-2'-O)-methyltransferase

Comments: The enzyme catalyses the methylation of guanosine18 in tRNA.

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 37257-01-5


1. Gefter, M.L. The in vitro synthesis of 2'-O-methylguanosine and 2-methylthio 6N (γ,γ-dimethylallyl) adenosine in transfer RNA of Escherichia coli. Biochem. Biophys. Res. Commun. 36 (1969) 435-441. [PMID: 4898378]

2. Kumagai, I., Watanabe, K. and Oshima, T. Thermally induced biosynthesis of 2'-O-methylguanosine in tRNA from an extreme thermophile, Thermus thermophilus HB27. Proc. Natl. Acad. Sci. USA 77 (1980) 1922-1926. [PMID: 6990416]

3. Hori, H., Yamazaki, N., Matsumoto, T., Watanabe, Y., Ueda, T., Nishikawa, K., Kumagai, I. and Watanabe, K. Substrate recognition of tRNA (guanosine-2'-)-methyltransferase from Thermus thermophilus HB27. J. Biol. Chem. 273 (1998) 25721-25727. [PMID: 9748240]

4. Pleshe, E., Truesdell, J. and Batey, R.T. Structure of a class II TrmH tRNA-modifying enzyme from Aquifex aeolicus. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 61 (2005) 722-728. [PMID: 16511140]

5. Ochi, A., Makabe, K., Kuwajima, K. and Hori, H. Flexible recognition of the tRNA G18 methylation target site by TrmH methyltransferase through first binding and induced fit processes. J. Biol. Chem. 285 (2010) 9018-9029. [PMID: 20053984]

[EC created 1972, modified 2005, modified 2011]

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