Reaction: S-adenosyl-L-methionine + a 3'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 3'-methoxyisoflavone
Other name(s): PlOMT4; OMT4; 3'-OMT
Systematic name: S-adenosyl-L-methionine:3'-hydroxyisoflavone 3'-O-methyltransferase
Comments: The enzyme catalyses a step in the biosynthesis of 3'-methoxypuerarin, an isoflavone with bioactive properties isolated from the roots of the leguminous plant Pueraria lobata var. leguminosa. The highest activity has been found for 3'-hydroxydaidzein. The enzyme has low activity on the 3'-hydroxyflavones luteolin and quercetin. 3'-hydroxypuerarin is not a substrate.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Li, J., Li, C., Gou, J. and Zhang, Y. Molecular cloning and functional characterization of a novel isoflavone 3'-O-methyltransferase from Pueraria lobata. Front. Plant Sci. 7 (2016) 793. [PMID: 27458460]