Reaction: carbamoyl phosphate + decarbamoylnovobiocin = phosphate + novobiocin
For diagram of reaction click here.
Glossary: decarbamoylnovobiocin = N-{7-[(6-deoxy-5-methyl-4-O-methyl-β-D-gulopyranosyl)oxy]4-hydroxy-8-methyl-2-oxo-2H-chromen-3-yl}-4-hydroxy-3-(3-methyl-2-buten-1-yl)benzamide
Other name(s): novN (gene name)
Systematic name: carbamoyl phosphate:decarbamoylnovobiocin 3''-O-carbamoyltransferase
Comments: The enzyme catalyses the last step in the biosynthesis of the aminocoumarin antibiotic novobiocin. The reaction is activated by ATP [1].
Links to other databases: BRENDA, EXPASY, KEGG Metacyc, CAS registry number:
References:
1. Freel Meyers, C.L., Oberthur, M., Xu, H., Heide, L., Kahne, D. and Walsh, C.T. Characterization of NovP and NovN: completion of novobiocin biosynthesis by sequential tailoring of the noviosyl ring. Angew. Chem. Int. Ed. Engl. 43 (2004) 67-70. [PMID: 14694473]
2. Gomez Garcia, I., Freel Meyers, C.L., Walsh, C.T. and Lawson, D.M. Crystallization and preliminary X-ray analysis of the O-carbamoyltransferase NovN from the novobiocin-biosynthetic cluster of Streptomyces spheroides. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 64 (2008) 1000-1002. [PMID: 18997325]