Reaction: a long-chain fatty acyl-CoA + α,α-trehalose = a 2-(long-chain-fatty acyl)-trehalose + CoA
Glossary: polyacyltrehalose = PAT = a 2-(long-chain fatty acyl)-2',3,4',6-tetramycolipenoyl-trehalose
a mycolipenoyl-CoA = a (2E,2S,4S,6S)-2,4,6-trimethyl-2-enoyl-CoA
Other name(s): papA3 (gene name)
Systematic name: long-chain fatty acyl-CoA:α,α-trehalose 2-acyltransferase
Comments: The enzyme, characterized from the bacterium Mycobacterium tuberculosis, participates in the biosynthesis of polyacyltrehalose (PAT), a pentaacylated, trehalose-based glycolipid found in the cell wall of pathogenic strains. The enzyme catalyses two successive activities - it first transfers an acyl (often palmitoyl) group to position 2, followed by the transfer of a mycolipenyl group to position 3 (see EC 188.8.131.528, mycolipenoyl-CoA2-(long-chain-fatty acyl)-trehalose mycolipenoyltransferase).
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
1. Hatzios, S.K., Schelle, M.W., Holsclaw, C.M., Behrens, C.R., Botyanszki, Z., Lin, F.L., Carlson, B.L., Kumar, P., Leary, J.A. and Bertozzi, C.R. PapA3 is an acyltransferase required for polyacyltrehalose biosynthesis in Mycobacterium tuberculosis. J. Biol. Chem 284 (2009) 12745-12751. [PMID: 19276083]