Reaction: 2 chlorogenate = 3,5-dicaffeoylquinate + quinate
Other name(s): CCT (gene name)
Systematic name: chlorogenate:chlorogenate 3-O-caffeoyltransferase
Comments: The enzyme has been purified from Solanum lycopersicum (tomato) fruits. In the vacuole, at pH 4-5, it produces 3,5-dicaffeoylquinic acid from cholorgenic acid. In the cytoplasm, at pH 6-7, it can use aromatic acyl-CoAs as donors and catalyses the reaction of EC 2.3.1.99 (quinate O-hydroxycinnamoyltransferase). The initial product, 3,5-dicaffeoylquinic acid, undergoes spontaneous acyl migration to form 4,5-dicaffeoylquinic acid. Small amounts of 1,5-dicaffeoylquinic acid are formed as a byproduct.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Moglia, A., Lanteri, S., Comino, C., Hill, L., Knevitt, D., Cagliero, C., Rubiolo, P., Bornemann, S. and Martin, C. Dual catalytic activity of hydroxycinnamoyl-coenzyme A quinate transferase from tomato allows it to moonlight in the synthesis of both mono- and dicaffeoylquinic acids. Plant Physiol. 166 (2014) 1777-1787. [PMID: 25301886]