IUBMB Enzyme Nomenclature


Accepted name: aminoglycoside 6'-N-acetyltransferase

Reaction: acetyl-CoA + kanamycin-B = CoA + N6'-acetylkanamycin-B

Glossary: kanamycin

Other name(s): aminoglycoside N6'-acetyltransferase; aminoglycoside-6'-acetyltransferase; aminoglycoside-6-N-acetyltransferase; kanamycin acetyltransferase

Systematic name: acetyl-CoA:kanamycin-B N6'-acetyltransferase

Comments: The antibiotics kanamycin A, kanamycin B, neomycin, gentamicin C1a, gentamicin C2 and sisomicin are substrates. The antibiotic tobramycin, but not paromomycin, can also act as acceptor. The 6-amino group of the purpurosamine ring is acetylated.

Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 56467-65-3


1. le Goffic, F. and Martel, A. La résistance aux aminosides provoquée par une isoenzyme la kanamycine acétyltransférase. Biochimie 56 (1974) 893-897. [PMID: 4614862]

2. Benveniste, R. and Davies, J.E. Enzymatic acetylation of aminoglycoside antibiotics by Escherichia coli carrying an R factor. Biochemistry 10 (1971) 1787-1796. [PMID: 4935296]

3. Dowding, J.E. Mechanisms of gentamicin resistance in Staphylococcus aureus. Antimicrob. Agents Chemother. 11 (1977) 47-50. [PMID: 836013]

[EC created 1976 as EC, transferred 1999 to EC, modified 1999, modified 2015]

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