Reaction: L-isoleucyl-[tRNAIle] + altemicidin = [tRNAIle] + SB-203207
Glossary: altemicidin = (4aR,6S,7R,7aS)-4-carbamoyl-6-hydroxy-2-methyl-7-[(2-sulamoylacetyl)amino]-4a,5,6,7a-tetrahydro-1H-cyclopenta[c]pyridine-7-carboxylate
SB-203207 = (sulfonamide-N)(L-isoleucyl)altemidicin = (4aR,6S,7R,7aS)-4-(carbamoyl)-7-({[(2S,3S)-2-amino-3-methylpentanoyl]aminosulphonyl}acetamido)-6-hydroxy-2-methyl-1,4a,5,6,7,7a-hexahydro-2H-cyclopenta[c]pyrindine-7-carboxylate
SB-203207 = (sulfonamide-N)(L-isoleucyl)altemidicin = 4-(aminocarbonyl)-7-[[(2-amino-3-methylpentanoyl)aminosulphonyl]acetamido]-2,4a,5,6,7,7a-hexahydro-6-hydroxy-2-methyl-1H-2- pyrindine-7-carboxylate
Other name(s): sbzA (gene name)
Systematic name: L-isoleucyl-[tRNAIle]:altemicidin L-isoleucyltransferase
Comments: The enzyme, isolated from the bacterium Streptomyces sp. NCIMB40513, catalyses the N-aminoacyl transfer of L-isoleucine from a charged isoleucine tRNA onto the primary sulfonamide group of the monoterpene alkaloid altemicidin.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Hu, Z., Awakawa, T., Ma, Z. and Abe, I. Aminoacyl sulfonamide assembly in SB-203208 biosynthesis. Nat. Commun. 10 (2019) 184. [PMID: 30643149]