Reaction: 2 UDP-glucose + delphinidin 3-O-(6"-O-malonyl)-β-D-glucoside = 2 UDP + delphinidin 3-O-(6"-O-malonyl)-β-D-glucoside-3',5'-di-O-β-D-glucoside (overall reaction)
(1a) UDP-glucose + delphinidin 3-O-(6"-O-malonyl)-β-D-glucoside = UDP + delphinidin 3-O-(6"-O-malonyl)-β-D-glucoside-3'-O-β-D-glucoside
(1b) UDP-glucose + delphinidin 3-O-(6"-O-malonyl)-β-D-glucoside-3'-O-β-D-glucoside = UDP + delphinidin 3-O-(6"-O-malonyl)-β-D-glucoside-3',5'-di-O-β-D-glucoside
For diagram of reaction click here.
Glossary: delphinidin 3-O-(6"-O-malonyl)-β-D-glucoside-3',5'-di-O-β-D-glucoside = ternatin C5.
Other name(s): UDP-glucose:anthocyanin 3',5'-O-glucosyltransferase; UA3'5’GZ
Systematic name: UDP-glucose:delphinidin 3-O-(6"-O-malonyl)-β-D-glucoside 3'-O-glucosyltransferase
Comments: Ternatins are a group of polyacetylated delphinidin glucosides that confer blue color to the petals of butterfly pea (Clitoria ternatea). This enzyme catalyses two reactions in the biosynthesis of ternatin C5: the conversion of delphinidin 3-O-(6"-O-malonyl)-β-D-glucoside to delphinidin 3-O-(6"-O-malonyl)-β-D-glucoside-3'-O-β-D-glucoside, followed by the conversion of the later to ternatin C5, by transferring two glucosyl groups in a stepwise manner [1].
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number:
References:
1. Kogawa, K., Kato, N., Kazuma, K., Noda, N. and Suzuki, M. Purification and characterization of UDP-glucose: anthocyanin 3',5'-O-glucosyltransferase from Clitoria ternatea. Planta 226 (2007) 1501-1509. [PMID: 17668234]