Reaction: sucrose + [(1→3)-α-D-glucosyl]n = D-fructose + [(1→3)-α-D-glucosyl]n+1
Other name(s): gtfJ (gene name)
Systematic name: sucrose:(1→3)-α-D-glucan 3-α-D-glucosyltransferase
Comments: The glucansucrases transfer a D-glusosyl residue from sucrose to a glucan chain. They are classified based on the linkage by which they attach the transferred residue. In some cases, in which the enzyme forms more than one linkage type, classification relies on the relative proportion of the linkages that are generated. Currently classified glucansucrases include EC 22.214.171.1242, mutansucrase, which extends (1→3)-α-D-glucans; EC 126.96.36.199, amylosucrase, which extends (1→4)-α-D-glucans; EC 188.8.131.52, dextransucrase, which extends (1→6) α-D-glucans; EC 184.108.40.2063, α-(1→2) branching sucrase, which introduces α(1→2) branches into (1→6)-α-D-glucans; EC 220.127.116.11, sucrose—1,6-α-glucan 3(6)-α-glucosyltransferase, which extends (1→6) α-D-glucans by both α(1→3) and α(1→6) linkages, with one of the linkage types being dominant; and EC 18.104.22.168, alternansucrase, which forms both α(1→3) and α(1→6) linkages in approximately equal amounts by alternating the linkage type.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
1. Simpson, C.L., Cheetham, N.W., Giffard, P.M. and Jacques, N.A. Four glucosyltransferases, GtfJ, GtfK, GtfL and GtfM, from Streptococcus salivarius ATCC 25975. Microbiology 141 (1995) 1451-1460. [PMID: 7545511]
2. Puanglek, S., Kimura, S., Enomoto-Rogers, Y., Kabe, T., Yoshida, M., Wada, M. and Iwata, T. In vitro synthesis of linear α-1,3-glucan and chemical modification to ester derivatives exhibiting outstanding thermal properties. Sci Rep 6 (2016) 30479. [PMID: 27469976]