Reaction: β-D-glucosyl-(1→4)-D-galactose + phosphate = α-D-glucopyranose 1-phosphate + D-galactopyranose
Other name(s): 4-O-β-D-glucosyl-D-galactose phosphorylase
Systematic name: β-D-glucosyl-(1→4)-D-galactose:phosphate α-D-glucosyltransferase (configuration-inverting)
Comments: The enzyme from the bacterium Paenibacillus polymyxa belongs to glycoside hydrolase family 94. It has a much lower activity with 4-O-β-D-glucosyl-L-arabinose.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. De Doncker, M., De Graeve, C., Franceus, J., Beerens, K., Kren, V., Pelantova, H., Vercauteren, R. and Desmet, T. Exploration of GH94 sequence space for enzyme discovery reveals a novel glucosylgalactose phosphorylase specificity. ChemBioChem (2021) . [PMID: 34541742]