Reaction: UDP-α-D-galactofuranose + a β-D-galactofuranoside = UDP + a β-D-Galf-(1→5)-β-D-galactofuranoside
Other name(s): gfsA (gene name); O-glycan β-1,5-galactofuranosyltransferase
Systematic name: UDP-α-D-galactofuranose:β-D-galactofuranoside β-1,5-galactofuranosyltransferase (configuration-inverting)
Comments: Requires Mn2+. The enzyme, characterized from the flilamentous fungi Aspergillus nidulans and Aspergillus fumigatus, participates in the synthesis of fungal-type galactomannan and O-mannose-type galactomannan, which are embedded in the fungal cell walls. While the reaction shown here describes the transfer of one galactofuransyl residue, the enzyme can add up to 5 more galactofuranosyl residues.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Komachi, Y., Hatakeyama, S., Motomatsu, H., Futagami, T., Kizjakina, K., Sobrado, P., Ekino, K., Takegawa, K., Goto, M., Nomura, Y. and Oka, T. GfsA encodes a novel galactofuranosyltransferase involved in biosynthesis of galactofuranose antigen of O-glycan in Aspergillus nidulans and Aspergillus fumigatus. Mol. Microbiol. 90 (2013) 1054-1073. [PMID: 24118544]
2. Katafuchi, Y., Li, Q., Tanaka, Y., Shinozuka, S., Kawamitsu, Y., Izumi, M., Ekino, K., Mizuki, K., Takegawa, K., Shibata, N., Goto, M., Nomura, Y., Ohta, K. and Oka, T. GfsA is a β1,5-galactofuranosyltransferase involved in the biosynthesis of the galactofuran side chain of fungal-type galactomannan in Aspergillus fumigatus. Glycobiology 27 (2017) 568-581. [PMID: 28369326]
3. Chihara, Y., Tanaka, Y., Izumi, M., Hagiwara, D., Watanabe, A., Takegawa, K., Kamei, K., Shibata, N., Ohta, K. and Oka, T. Biosynthesis of β-(1-→5)-Galactofuranosyl Chains of Fungal-Type and O-Mannose-Type Galactomannans within the Invasive Pathogen Aspergillus fumigatus. mSphere 5 (2020) . [PMID: 31941812]