Reaction: dolichyl β-D-mannosyl phosphate + L-tryptophyl-[protein] = dolichyl phosphate + 2-C-(α-D-mannosyl)-L-tryptophyl-[protein]
Other name(s): Dpy-19; dumpy-19; DPY19L1; DPY19L3
Systematic name: dolichyl β-D-mannosyl-phosphate:L-tryptophyl-[protein] 2-C-D-mannosyltransferase (configuration-inverting)
Comments: This enzyme transfers a mannose to the first tryptophan in a WxxW or WxxC consensus sequon of acceptor proteins and is related to the oligosaccharyltransferase family involved in protein N-glycosylation, which utilizes a dolichol diphosphate-linked oligosaccharide as the donor (EC 2.4.99.18, dolichyl-diphosphooligosaccharide—protein glycotransferase). Enzymes from Caenorhabditis elegans and mammals have been characterized.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Buettner, F.F., Ashikov, A., Tiemann, B., Lehle, L. and Bakker, H. C. elegans DPY-19 is a C-mannosyltransferase glycosylating thrombospondin repeats. Mol. Cell 50 (2013) 295-302. [PMID: 23562325]
2. Shcherbakova, A., Tiemann, B., Buettner, F.F. and Bakker, H. Distinct C-mannosylation of netrin receptor thrombospondin type 1 repeats by mammalian DPY19L1 and DPY19L3. Proc. Natl. Acad. Sci. USA 114 (2017) 2574-2579. [PMID: 28202721]
3. Bloch, J.S., John, A., Mao, R., Mukherjee, S., Boilevin, J., Irobalieva, R.N., Darbre, T., Scott, N.E., Reymond, J.L., Kossiakoff, A.A., Goddard-Borger, E.D. and Locher, K.P. Structure, sequon recognition and mechanism of tryptophan C-mannosyltransferase. Nat. Chem. Biol. 19 (2023) 575-584. [PMID: 36604564]