Reaction: NAD+ + glycine + sulfide = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H2O
Other name(s): Thi4 (ambiguous)
Systematic name: NAD+:glycine ADP-D-ribosyltransferase (sulfide-adding)
Comments: This iron dependent enzyme, found in most archaea, is involved in the biosynthesis of thiamine phosphate. The homologous enzyme from plants and fungi (EC 188.8.131.52, cysteine-dependent adenosine diphosphate thiazole synthase) uses an intrinsic cysteine as sulfur donor and, unlike the archaeal enzyme, is a single turn-over enzyme.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
1. Zhang, X., Eser, B.E., Chanani, P.K., Begley, T.P. and Ealick, S.E. Structural basis for iron-mediated sulfur transfer in archael and yeast thiazole synthases. Biochemistry 55 (2016) 1826-1838. [PMID: 26919468]
2. Eser, B.E., Zhang, X., Chanani, P.K., Begley, T.P. and Ealick, S.E. From suicide enzyme to catalyst: the iron-dependent sulfide transfer in Methanococcus jannaschii thiamin thiazole biosynthesis. J. Am. Chem. Soc. 138 (2016) 3639-3642. [PMID: 26928142]