Reaction: deamido-NAD+ + hydrogencarbonate = AMP + pyridinium-3,5-biscarboxylate mononucleotide
Other name(s): LarB; P2CMN synthase; nicotinic acid adenine dinucleotide carboxylase/hydrolase; NaAD carboxylase/hydrolase
Systematic name: deamido-NAD+:hydrogencarbonate nicotinate-β-D-ribonucleotidyltransferase
Comments: This enzyme, found in the bacterium Lactobacillus plantarum, is involved in the biosynthesis of a nickel-pincer cofactor. It carboxylates the pyridinium ring of deamido-NAD+ and cleaves the phosphoanhydride bond to release AMP and generate pyridinium-3,5-biscarboxylic acid mononucleotide (P2CMN).
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number:
1. Desguin, B., Soumillion, P., Hols, P. and Hausinger, R.P. Nickel-pincer cofactor biosynthesis involves LarB-catalyzed pyridinium carboxylation and LarE-dependent sacrificial sulfur insertion. Proc. Natl Acad. Sci. USA 113 (2016) 5598-5603. [PMID: 27114550]