IUBMB Enzyme Nomenclature


Accepted name: tRNA dimethylallyltransferase

Reaction: prenyl diphosphate + adenine37 in tRNA = diphosphate + N6-prenyladenine37 in tRNA

For diagram of reaction click here.

Other name(s): tRNA prenyltransferase, MiaA, transfer ribonucleate isopentenyltransferase (incorrect); Δ2-isopentenyl pyrophosphate:tRNA-Δ2-isopentenyl transferase (incorrect); Δ2-isopentenyl pyrophosphate:transfer ribonucleic acid Δ2-isopentenyltransferase (incorrect); dimethylallyl-diphosphate: tRNA dimethylallyltransferase; dimethylallyl-diphosphate:adenine37 in tRNA dimethylallyltransferase

Systematic name: prenyl-diphosphate:adenine37 in tRNA dimethylallyltransferase

Comments: Formerly known as tRNA isopentenyltransferase EC, but it is now known that dimethyallyl diphosphate, rather than isopentenyl diphosphate, is the substrate.

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number:


1. Leung, H.C., Chen, Y. and Winkler, M.E. Regulation of substrate recognition by the MiaA tRNA prenyltransferase modification enzyme of Escherichia coli K-12. J. Biol. Chem. 272 (1997) 13073-13083. [PMID: 9148919]

2. Soderberg, T. and Poulter, C.D. Escherichia coli dimethylallyl diphosphate:tRNA dimethylallyltransferase: essential elements for recognition of tRNA substrates within the anticodon stem-loop. Biochemistry 39 (2000) 6546-6553. [PMID: 10828971]

3. Moore, J.A., Mathis, J.R. and Poulter, C.D. Escherichia coli dimethylallyl diphosphate:tRNA dimethylallyltransferase: pre-steady-state kinetic studies. Biochim. Biophys. Acta 1479 (2000) 166-174. [PMID: 11004538]

[EC created 1972 as EC, transferred 2009 to EC]

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