IUBMB Enzyme Nomenclature


Accepted name: tRNA-uridine 2-sulfurtransferase

Reaction: a [protein]-S-sulfanyl-L-cysteine + uridine34 in tRNA + ATP + reduced acceptor = a [protein]-L-cysteine + 2-thiouridine34 in tRNA + AMP + diphosphate + acceptor

Other name(s): mnmA (gene name)

Systematic name: [protein]-S-sulfanyl-L-cysteine:tRNA (uridine34-2-O)-sulfurtransferase

Comments: The enzyme, found in bacteria, catalyses formation of the 2-thiouridine modification in the wobble position of tRNAGln, tRNALys and tRNAGlu.

Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number:


1. Kambampati, R. and Lauhon, C.T. MnmA and IscS are required for in vitro 2-thiouridine biosynthesis in Escherichia coli. Biochemistry 42 (2003) 1109-1117. [PMID: 12549933]

2. Ikeuchi, Y., Shigi, N., Kato, J., Nishimura, A. and Suzuki, T. Mechanistic insights into sulfur relay by multiple sulfur mediators involved in thiouridine biosynthesis at tRNA wobble positions. Mol. Cell 21 (2006) 97-108. [PMID: 16387657]

[EC created 2015]

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