Reaction: 3'-phosphoadenylyl sulfate + 5α-cholan-3α,7α,12α,24-tetrol = adenosine 3',5'-bisphosphate + 5α-cholan-3α,7α,12α-triol 24-sulfate
For diagram click here.
Glossary: petromyzonol = 5α-cholan-3α,7α,12α,24-tetrol
3'-phosphoadenylyl sulfate = PAPS
Other name(s): PZ-SULT; 3'-phosphoadenylyl-sulfate:5α-cholan-3α,7α,12α,24-tetrol sulfotransferase
Systematic name: 3'-phosphoadenylyl-sulfate:5α-cholan-3α,7α,12α,24-tetrol sulfonotransferase
Comments: The enzyme from the lamprey Petromyzon marinus can also use the corresponding 3-ketone as a substrate. It is stereoselective (5α-cholane) and regioselective, exhibiting a preference for an hydroxy group at C-24. The enzyme is inactive when allocholic acid, which has a carboxy group at C-24, is used as a substrate.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number:
References:
1. Venkatachalam, K.V., Llanos, D.E., Karami, K.J. and Malinovskii, V.A. Isolation, partial purification, and characterization of a novel petromyzonol sulfotransferase from Petromyzon marinus (lamprey) larval liver. J. Lipid Res. 45 (2004) 486-495. [PMID: 14657197]