Reaction: 3'-phosphoadenylyl sulfate + chondroitin = adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
For diagram click here.
Glossary: 3'-phosphoadenylyl sulfate = PAPS
Other names: chondroitin sulfotransferase; 3'-phosphoadenylyl-sulfate:chondroitin 4'-sulfotransferase
Systematic name: 3'-phosphoadenylyl-sulfate:chondroitin 4'-sulfonotransferase
Comments: The sulfation takes place at the 4-position of N-acetyl-galactosamine residues of chondroitin. Not identical with EC 2.8.2.17 chondroitin 6-sulfotransferase.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 83589-04-2
References:
1. Habuchi, O. and Miyashita, N. Separation and characterization of chondroitin 6-sulfotransferase and chondroitin 4-sulfotransferase from chick embryo cartilage. Biochim. Biophys. Acta 717 (1982) 414-421. [PMID: 6957247]
2. Nakanishi, Y., Otsu, K. and Suzuki, S. Enzymatic transfer of galactosyl phosphate from UDP-galactose to UDP-N-acetylglucosamine. FEBS Lett. 151 (1983) 15-18. [PMID: 6130977]
3. Nakanishi, Y., Shimizu, M., Otsu, K., Kato, S., Tsuji, M. and Suzuki, S. A terminal 6-sulfotransferase catalyzing a synthesis of N-acetylgalactosamine 4,6-bissulfate residue at the nonreducing terminal position of chondroitin sulfate. J. Biol. Chem. 256 (1981) 5443-5449. [PMID: 6787041]
4. Suzuki, S. and Strominger, J.L. Enzymatic sulfation of mucopolysaccharides in hen oviduct. I. Transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to mucopolysaccharides. J. Biol. Chem. 235 (1960) 257-266.
5. Suzuki, S. and Strominger, J.L. Enzymatic sulfation of mucopolysaccharides in hen oviduct. II. Mechanism of the reaction studied with oligosaccharides and monosaccharides as acceptors. J. Biol. Chem. 235 (1960) 267-273.
6. Suzuki, S. and Strominger, J.L. Enzymatic sulfation of mucopolysaccharides in hen oviduct. III. Mechanism of sulfation of chondroitin and chondroitin sulfate A. J. Biol. Chem. 235 (1960) 274-276.