IUBMB Enzyme Nomenclature


Accepted name: crossover junction endodeoxyribonuclease

Reaction: Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction)

Other name(s): Hje endonuclease; Holliday junction endonuclease CCE1; Holliday junction resolvase; Holliday junction-cleaving endonuclease; Holliday junction-resolving endoribonuclease; RusA Holliday junction resolvase; RusA endonuclease; RuvC endonuclease; SpCCe1 Holliday junction resolvase; crossover junction endoribonuclease; cruciform-cutting endonuclease; endo X3; endonuclease RuvC; endonuclease VII; endonuclease X3; resolving enzyme CCE1

Comments: The enzyme from Saccharomyces cerevisiae has no endonuclease or exonuclease activity on single-stranded or double-stranded DNA molecules that do not contain Holliday junctions.

Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:


1. Symington, L.S. and Kolodner, R. Partial purification of an enzyme from Saccharomyces cerevisiae that cleaves Holliday junctions. Proc. Natl. Acad. Sci. USA 82 (1985) 7247-7251. [PMID: 3903750]

2. Shida, T., Iwasaki, H., Saito, A., Kyogoku, Y. and Shinagawa, H. Analysis of substrate specificity of the RuvC holliday junction resolvase with synthetic Holliday junctions. J. Biol. Chem. 271 (1996) 26105-26109. [PMID: 8824253]

3. Shah, R., Cosstick, R. and West, S.C. The RuvC protein dimer resolves Holliday junctions by a dual incision mechanism that involves base-specific contacts. EMBO J. 16 (1997) 1464-1472. [PMID: 9135161]

4. Fogg, J.M., Schofield, M.J., White, M.F. and Lilley, D.M. Sequence and functional-group specificity for cleavage of DNA junctions by RuvC of Escherichia coli. Biochemistry 38 (1999) 11349-11358. [PMID: 10471285]

5. Lilley, D.M. and White, M.F. The junction-resolving enzymes. Nat. Rev. Mol. Cell. Biol. 2 (2001) 433-443. [PMID: 11389467]

6. Middleton, C.L., Parker, J.L., Richard, D.J., White, M.F. and Bond, C.S. Crystallization and preliminary X-ray diffraction studies of Hje, a Holliday junction resolving enzyme from Sulfolobus solfataricus. Acta Crystallogr. D Biol. Crystallogr. 59 (2003) 171-173. [PMID: 12499561]

[EC created 1989 as EC, modified 2003, transferred 2021 to EC]

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