Reaction: endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule
Other name(s): 3 tRNase; tRNA 3 endonuclease; RNase Z; 3' tRNase
Comments: No cofactor requirements. An homologous enzyme to that found in Arabidopsis thaliana has been found in Methanococcus janaschii.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 98148-84-6
References:
1. Schiffer, S., Rösch, S. and Marchfelder, A. Assigning a function to a conserved group of proteins: the tRNA 3'-processing enzymes. EMBO J. 21 (2002) 2769-2777. [PMID: 12032089]
2. Mayer, M., Schiffer, S. and Marchfelder, A. tRNA 3' processing in plants: nuclear and mitochondrial activities differ. Biochemistry 39 (2000) 2096-2105. [PMID: 10684660]
3. Schiffer, S., Helm, M., Theobald-Dietrich, A., Giege, R. and Marchfelder, A. The plant tRNA 3' processing enzyme has a broad substrate spectrum. Biochemistry 40 (2001) 8264-8272. [PMID: 11444972]
4. Kunzmann, A., Brennicke, A. and Marchfelder, A. 5' end maturation and RNA editing have to precede tRNA 3' processing in plant mitochondria. Proc. Natl. Acad. Sci. USA 95 (1998) 108-113. [PMID: 9419337]
5. Mörl, M. and Marchfelder, A. The final cut. The importance of tRNA 3'-processing. EMBO Rep. 2 (2001) 17-20. [PMID: 11252717]
6. Minagawa, A., Takaku, H., Takagi, M. and Nashimoto, M. A novel endonucleolytic mechanism to generate the CCA 3' termini of tRNA molecules in Thermotoga maritima. J. Biol. Chem. 279 (2004) 15688-15697.[PMID: 14749326]
7. Takaku, H., Minagawa, A., Takagi, M. and Nashimoto, M. A candidate prostate cancer susceptibility gene encodes tRNA 3' processing endoribonuclease. Nucleic Acids Res. 31 (2003) 2272-2278. [PMID: 12711671]