IUBMB Enzyme Nomenclature


Accepted name: RNA 2',3'-cyclic 3'-phosphodiesterase

Reaction: (ribonucleotide)n-2',3'-cyclic phosphate + H2O = (ribonucleotide)n-2'-phosphate

Other name(s): thpR (gene name); ligT (gene name)

Systematic name: (ribonucleotide)n-2',3'-cyclic phosphate 3'-nucleotidohydrolase

Comments: The enzyme hydrolyses RNA 2',3'-cyclic phosphodiester to an RNA 2'-phosphomonoester. In vitro the enzyme can also ligate tRNA molecules with 2',3'-cyclic phosphate to tRNA with 5'-hydroxyl termini, forming a 2'-5' phosphodiester linkage. However, the ligase activity is unlikely to be relevant in vivo.

Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number:


1. Kanai, A., Sato, A., Fukuda, Y., Okada, K., Matsuda, T., Sakamoto, T., Muto, Y., Yokoyama, S., Kawai, G. and Tomita, M. Characterization of a heat-stable enzyme possessing GTP-dependent RNA ligase activity from a hyperthermophilic archaeon, Pyrococcus furiosus. RNA 15 (2009) 420-431. [PMID: 19155324]

2. Remus, B.S., Jacewicz, A. and Shuman, S. Structure and mechanism of E. coli RNA 2',3'-cyclic phosphodiesterase. RNA 20 (2014) 1697-1705. [PMID: 25239919]

[EC created 2017]

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