Reaction: Hydrolysis of terminal, non-reducing β-D-galactose residues in (1→3)-β-D-galactopyranans
Systematic name: galactan 3-β-D-galactosidase
Comments: This enzyme removes not only free galactose, but also 6-glycosylated residues, e.g., (1→6)-β-D-galactobiose, and galactose bearing oligosaccharide chains on O-6. Hence, it releases branches from [arabino-galacto-(1→6)]-(1→3)-β-D-galactans.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 161515-48-6
References:
1. Tsumuraya, Y., Mochizuki, N., Hashimoto Y. and Kovac, P. Purification of exo-(1→3)-D-galactanase of Irpex lacteus (Polyporus tulipiferae) and its action on arabinogalactan-proteins. J. Biol. Chem. 265 (1990) 7207-7215. [PMID: 2158993]
2. Pellerin, P. and Brillouet, J.M. Purification and properties of an exo-(1→3)-β-D-galactanase from Aspergillus niger. Carbohydr. Res. 264 (1994) 281-291. [PMID: 7805066]