IUBMB Enzyme Nomenclature


Accepted name: mannosylglycoprotein endo-β-mannosidase

Reaction: Hydrolysis of the α-D-mannosyl-(1→6)-β-D-mannosyl-(1→4)-N-acetyl-β-D-glucosaminyl-(1→4)-N-acetyl-β-D-glucosaminyl sequence of glycoprotein to α-D-mannosyl-(1→6)-D-mannose and N-acetyl-β-D-glucosaminyl-(1→4)-N-acetyl-β-D-glucosaminyl sequences

Other name(s): endo-β-mannosidase

Comments: The substrate group is a substituent on N-4 of an asparagine residue in the glycoprotein. The mannose residue at the non-reducing end of the sequence may carry further α-D-mannosyl groups on O-3 or O-6, but such a substituent on O-3 of the β-D-mannosyl group prevents the action of the enzyme. The enzyme was obtained from the lily, Lilium longiflorum.

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 141176-95-6


1. Ishimizu, T., Sasaki, A., Okutani, S., Maeda, M., Yamagishi, M. and Hase, S. Endo-β-mannosidase, a plant enzyme acting on N-glycan. Purification, molecular cloning, and characterization. J. Biol. Chem. 279 (2004) 38555-38562. [PMID: 15247239]

2. Sasaki, A., Yamagishi, M., Mega, T., Norioka, S., Natsuka, S. and Hase, S. Partial purification and characterization of a novel endo-β-mannosidase acting on N-linked sugar chains from Lilium longiflorum thumb. J. Biochem. (Tokyo) 125 (1999) 363-367. [PMID: 9990135]

[EC created 2005]

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