Reaction: Endohydrolysis of β-(1→4)-linkages between β-D-galactopyranose-6-sulfate and 3,6-anhydro-α-L-galactopyranose units in funoran
Glossary: funoran = [→3)-β-D-galactopyranose-6-sulfate-(1→4)-3,6-anhydro-α-L-galactopyranose-(1→]
Other name(s): β-funoranase
Systematic name: funoran endo β-(1,4)-glycanohydrolase
Comments: The enzyme is an endo hydrolase that hydrolyses the β(1→4) bond in funoran, a polysaccharide produced by red algae of the genus Gloiopeltis. The enzyme from the marine bacterium Wenyingzhuangia aestuarii OF219 acts on agarose with a higher efficiency (cf. EC 3.2.1.81, β-agarase), but binds funoran preferentially.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Zhang, Y., Chen, G., Shen, J., Mei, X., Liu, G., Chang, Y., Dong, S., Feng, Y., Wang, Y. and Xue, C. The characteristic structure of funoran could be hydrolyzed by a GH86 family enzyme (Aga86A_Wa): Discovery of the funoran hydrolase. Carbohyd Polym 318 (2023) 121117.