Reaction: Hydrolysis of terminal non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides
Other name(s): invertase; saccharase; glucosucrase; β-h-fructosidase; β-fructosidase; invertin; sucrase; maxinvert L 1000; fructosylinvertase; alkaline invertase; acid invertase
Systematic name: β-D-fructofuranoside fructohydrolase
Comments:The enzyme, found in bacteria, plants, and fungi, was discovered in yeast as an enzyme that hydrolyses sucrose. It was originally called invertase due to the fact that when sucrose is hydrolysed, the optical rotation reverts from dextrorotatory to levorotatory. The enzyme recognizes and hydrolyses the β-fructofuranoside side of the sucrose molecule and has low specificity regarding the other end of the molecule (a different enzyme, EC 3.2.1.48, sucrose α-glucosidase, recognizes and hydrolyses the glucoside group on the opposite side of sucrose.) Some, but not all, enzymes can act on short fructooligosaccharides such as 1-kestotrisoe, 1,1-kestotetraose, raffinose and stachiose. The enzyme also catalyses fructotransferase reactions.
Links to other databases: BRENDA, EXPASY, GTD, KEGG, Metacyc, PDB, CAS registry number: 9001-57-4
References:
1. Myrbäck, K. Invertases, in Boyer, P.D., Lardy, H. and Myrbäck, K. (Eds.), The Enzymes, 2nd edn., vol. 4, Academic Press, New York, 1960, pp. 379-396.
2. Neumann, N.P. and Lampen, J.O. Purification and properties of yeast invertase. Biochemistry 6 (1967) 468-475. [PMID: 4963242]