EC 3.4.17.14

Reaction: Cleavage of the bond: (Ac)₂-L-lysyl-D-alanylD-alanine

Other names: Zn²⁺ G peptidase; D-alanyl-D-alanine hydrolase; D-alanyl-D-alanine-cleaving carboxypeptidase; DD-carboxypeptidase; G enzyme; DD-carboxypeptidase-transpeptidase

Comments: A zinc enzyme. Catalyzes carboxypeptidation but not transpeptidation reactions involved in bacterial cell wall metabolism. Weakly inhibited by β-lactams. In peptidase family M15. Distinct from EC 3.4.16.4, serine-type D-Ala-D-Ala carboxypeptidase.

Links to other databases: BRENDA, EXPASY, KEGG, MEROPS, Metacyc, PDB, CAS registry number: 213189-85-6

References

1. Dideberg, O., Charlier, P., Dive, G., Joris, B., Frère, J.M. and Ghuysen, J.M. Structure of a Zn²⁺-containing D-alanyl-D-alanine-cleaving carboxypeptidase at 2.5 Å resolution. Nature 299 (1982) 469-470. [PMID: 7121588]

2. Joris, B., Van Beeumen, J., CASagrande, F., Gerday, C., Frère, J.-M. and Ghuysen, J.-M. The complete amino acid sequence of the Zn²⁺-containing D-alanyl-D-alanine-cleaving carboxypeptidase of Streptomyces albus G. Eur. J. Biochem. 130 (1983) 53-69[PMID: 6825689]

3. Ghuysen, J.-M., Frère, J.-M., Leyh-Bouille, M., Nguyen-Disteche, M., Coyette, J., Dusart, J., Joris, B., Duez, C., Dideberg, O., Charlier, P., Dive, G. and Lamotte-Brasseur, J. Bacterial wall peptidoglycan, DD-peptidases and beta-lactam antibiotics. Scand. J. Infect. Dis. Suppl. 42 (1984) 17-37. [PMID: 6597561]