pair in the N-terminus, to yield mature renin
Reaction: Hydrolyses mouse Ren2 protein (a species of prorenin present in the submandibular gland) on the carboxy side of the arginine residue at the Lys-Arg pair in the N-terminus, to yield mature renin
Other name(s): KLK13; kallikrein mK13; mGK-13; mK13; mKLK13; prorenin converting enzyme 1; PRECE-1; prorenin-converting enzyme; PRECE; proteinase P
Comments: The enzyme is specific for prorenin from the mouse submandibular gland, as prorenin from the mouse kidney (Ren1) and human prorenin are not substrates [1]. Site-directed mutagenesis studies have shown that the enzyme will also cleave prorenin when Lys-Arg is replaced by Arg-Arg or Gln-Arg but the rate of reaction is much slower when Lys-Lys is used. This enzyme is also able to process pro-interleukin-1β (pro-IL-1β) in mouse submandibular gland to form IL-1β [4]. Belongs in peptidase family S1A.
Links to other databases: BRENDA, EXPASY, KEGG, MEROPS, Metacyc, PDB, CAS registry number: 342900-44-1
References:
1. Nakayama, K., Kim, W.S., Nakagawa, T., Nagahama, M. and Murakami, K. Substrate specificity of prorenin converting enzyme of mouse submandibular gland. Analysis using site-directed mutagenesis. J. Biol. Chem. 265 (1990) 21027-21031. [PMID: 2250008]
2. Kim, W.S., Hatsuzawa, K., Ishizuka, Y., Hashiba, K., Murakami, K. and Nakayama, K. A processing enzyme for prorenin in mouse submandibular gland. Purification and characterization. J. Biol. Chem. 265 (1990) 5930-5933. [PMID: 2180937]
3. Kikkawa, Y., Yamanaka, N., Tada, J., Kanamori, N., Tsumura, K. and Hosoi, K. Prorenin processing and restricted endoproteolysis by mouse tissue kallikrein family enzymes (mK1, mK9, mK13, and mK22). Biochim. Biophys. Acta 1382 (1998) 55-64. [PMID: 9507064]
4. Yao, C., Karabasil, M.R., Purwanti, N., Li, X., Akamatsu, T., Kanamori, N. and Hosoi, K. Tissue kallikrein mK13 is a candidate processing enzyme for the precursor of interleukin-1β in the submandibular gland of mice. J. Biol. Chem. 281 (2006) 7968-7976. [PMID: 16423834]