IUBMB Enzyme Nomenclature


Accepted name: cucumisin

Reaction: Hydrolysis of proteins with broad specificity

Other name(s): euphorbain; solanain; hurain; tabernamontanain

Comments: From the sarcocarp of the musk melon (Cucumis melo). In peptidase family S8 (subtilisin family). Other endopeptidases from plants, which are less well characterized but presumably of serine-type, include euphorbain from Euphorbia cerifera [6], solanain from horse-nettle Solanum elaeagnifolium [1], hurain from Hura crepitans [2] and tabernamontanain from Tabernamontana grandiflora [3].

Links to other databases: BRENDA, EXPASY, KEGG, MEROPS, Metacyc, PDB, CAS registry number: 82062-89-3


1. Greenberg, D.M. and Winnick, T. Plant proteases. I. Activation-inhibition reactions. J. Biol. Chem. 135 (1940) 761-773

2. Jaffé, W.G. Hurain, a new plant protease from Hura crepitans. J. Biol. Chem. 149 (1943) 1-7

3. Jaffé, W.G. A new vegetable proteolytic enzyme of the papain class. Rev. Brasil Biol. 3 (1943) 149-157

4. Kaneda, M. and Toninaga, N. Isolation and characterization of a proteinase from the sarcocarp of melon fruit. J. Biochem. (Tokyo) 78 (1975) 1287-1296. [PMID: 5423]

5. Kaneda, M., Ohmine, H., Yonezawa, H. and Tominaga, N. Amino acid sequence around the reactive site of cucumisin from melon fruit. J. Biochem. (Tokyo) 95 (1984) 825-829. [PMID: 6427203]

6. Lynn, K.R. and Clevette-Radford, N.A. Two proteases from the latex of Elaeophorbia drupifera. Phytochemistry 24 (1985) 2843-2845

7. Kaneda, N., Minematsu, Y., Powers, J.C. and Tominaga, N. Specificity of cucumisin in hydrolysis of peptide thiobenzyl esters. Agric. Biol. Chem. 50 (1986) 1075-1076

[EC created 1978 (EC created 1972 as EC transferred 1989 to EC, deleted 1992; EC created 1972 deleted 1992; EC created 1972 deleted 1992; EC created 1972 deleted 1992; all covered by EC]

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