Reaction: Hydrolysis of proteins with broad specificity, and of Bz-Arg-OEt > Ac-Tyr-OEt. Does not hydrolyze peptide amides
Other names: yeast proteinase B; proteinase yscB; baker's yeast proteinase B; brewer's yeast proteinase; peptidase β
Comments: From Saccharomyces cerevisiae (baker's yeast, brewer's yeast). In peptidase family S8 (subtilisin family), but contains a Cys residue near the active site His, and is inhibited by mercurials. Proteinase ycaB is a similar enzyme from the yeast Candida albicans [3]
Links to other databases: BRENDA, EXPASY, KEGG, MEROPS, Metacyc, CAS registry number: 37288-81-6
References
1. Félix, F. and Brouillet, N. Purification et propriétés de deux peptidases de levure de brassérie. Biochim. Biophys. Acta 122 (1966) 127-144. [PMID: 4961236]
2. Kominami, E., Hoffschulte, H., Leuschel, L., Maier, K. and Holzer, H. The substrate specificity of proteinase B from baker's yeast. Biochim. Biophys. Acta. 661 (1981) 136-141. [PMID: 7028121]
3. Farley, P.C., Shepherd, M.G. and Sullivan, P.A. The purification and properties of yeast proteinase B from Candida albicans. Biochem. J. 236 (1986) 177-184. [PMID: 3539100]
4. Moehle, C.M., Tizard, R., Lemmon, S.K., Smart, J. and Jones, E.W. Protease B of the lysosome like vacuole of the yeast Saccharomyces cerevisiae is homologous to the subtilisin family of serine proteases. Mol. Cell. Biol. 7 (1987) 4390-4399. [PMID: 3325823]