Ala bond leaving an N-terminal (75%) and a C-terminal (25%) fragment
Reaction: Hydrolysis of proteins including native collagen at Ala bond leaving an N-terminal (75%) and a C-terminal (25%) fragment
Other names: Hypoderma collagenase
Comments: From the larva of a warble fly, Hypoderma lineatum. Little action on small molecule substrates of trypsin, chymotrypsin, elastase or microbial collagenases. In peptidase family S1 (trypsin family)
Links to other databases: BRENDA, EXPASY, KEGG, MEROPS, Metacyc, PDB, CAS registry number: 122191-36-0
References
1. Lecroisey, A., Boulard, C. and Keil, B. Chemical and enzymatic characterization of the collagenase from the insect Hypoderma lineatum. Eur. J. Biochem. 101 (1979) 385-393. [PMID: 230030]
2. Lecroisey, A. and Keil, B. Specificity of the collagenase from the insect Hypoderma lineatum. Eur. J. Biochem. 152 (1985) 123-130. [PMID: 2995028]
3. Lecroisey, A., Gilles, A.-M., De Wolf, A. and Keil, B. Complete amino acid sequence of the collagenase from the insect Hypoderma lineatum. J. Biol. Chem. 262 (1987) 7546-7551. [PMID: 3034899]