EC 3.4.21.73

Reaction: Specific cleavage of ArgVal bond in plasminogen to form plasmin

Other names: urokinase; urinary plasminogen activator; cellular plasminogen activator; urokinase-type plasminogen activator; double-chain urokinase-type plasminogen activator; two-chain urokinase-type plasminogen activator; urokinase plasminogen activator; uPA; u-PA; abbokinase; urinary esterase A

Comments: Formed from the inactive precursor by action of plasmin or plasma kallikrein. Differs in structure from t-plasminogen activator (EC 3.4.21.68), and does not bind to fibrin. In peptidase family S1 (trypsin family). Formerly included in EC 3.4.21.31 and EC 3.4.99.26

Links to other databases: BRENDA, EXPASY, KEGG, MEROPS, Metacyc, PDB, CAS registry number: 9039-53-6

References

1. Lottenberg, R., Christiansen, U., Jackson, C.M. and Coleman, P.L. Assay of coagulation proteases using peptide chromogenic and fluorogenic substrates. Methods Enzymol. 80 (1981) 341-361. [PMID: 6210826]

2. Loskutoff, D.J. and Schleef, R.R. Plasminogen activators and their inhibitors. Methods Enzymol. 163 (1988) 293-302. [PMID: 3148826]

3. Saksela, O. and Rifkin, D.B. Cell-associated plasminogen activation: regulation and physiological functions. Annu. Rev. Cell Biol. 4 (1988) 93-126. [PMID: 3143380]

4. Collen, D., Lijnen, H.R. and Verstraete, M. The fibrinolytic system and its disorders. In Blood: Principles and Practice of Hematology, 2nd edn (Handin, R.I., Lux, S.E. and Stossel, J.P., eds), J.B.Lippincott Company, Philadelphia (1990)

5. Lijnen, H.R., Van Hoef, B., Nelles, L. and Collen, D. Plasminogen activation with single-chain urokinase-type plasminogen activator (scu-PA). Studies with active site mutagenized plasminogen (Ser⁷⁴⁰→Ala) and plasmin-resistant scu-PA (Lys¹⁵⁸→Glu). J. Biol. Chem. 265 (1990) 5232-5236. [PMID: 1969415]