IUBMB Enzyme Nomenclature


Accepted name: picornain 3C

Reaction: Selective cleavage of GlnGly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly

Other names: picornavirus endopeptidase 3C; poliovirus protease 3C; rhinovirus protease 3C; foot-and-mouth protease 3C; poliovirus proteinase 3C; rhinovirus proteinase 3C; coxsackievirus 3C proteinase; foot-and-mouth-disease virus proteinase 3C; 3C protease; 3C proteinase; cysteine proteinase 3C; hepatitis A virus 3C proteinase; protease 3C; tomato ringspot nepovirus 3C-related protease

Comments: From entero-, rhino-, aphto- and cardioviruses. Larger than the homologous virus picornain 2A. Type example of peptidase family C3

Links to other databases: BRENDA, EXPASY, KEGG, MEROPS, Metacyc, PDB, CAS registry number: 97162-88-4


1. Ivanoff, L.A., Towatari, T., Ray, J., Korant, B.D. and Petteway, S.R. Expression and site-specific mutagenesis of the poliovirus 3C protease in Escherichia coli. Proc. Natl. Acad. Sci. USA 83 (1986) 5392-5396. [PMID: 3016701]

2. Bazan, J.F. and Fletterick, R.J. Viral cysteine proteases are homologous to the trypsin-like family of serine proteases: structural and functional implications. Proc. Natl. Acad. Sci. USA 85 (1988) 7872-7876. [PMID: 3186696]

3. Kräusslich, H.-G. and Wimmer, E. Viral proteinases. Annu. Rev. Biochem. 57 (1988) 701-754. [PMID: 3052288]

4. Nicklin, M.J.H., Harris, K.S., Pallai, P.V. and Wimmer, E. Poliovirus proteinase 3C: large-scale expression, purification, and specific cleavage activity on natural and synthetic substrates in vitro. J. Virol. 62 (1988) 4586-4593. [PMID: 2846872]

[EC created 1992]

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