NHMec amongst small molecule substrates
Reaction: Broad specificity for cleavage of proteins, but strong preference for Z-Arg-ArgNHMec amongst small molecule substrates
Other names: bromelain; pineapple stem bromelain
Comments: The most abundant of the cysteine endopeptidases of the stem of the pineapple plant, Ananas comosus. Distinct from the bromelain found in the pineapple fruit (EC 3.4.22.33). Scarcely inhibited by chicken cystatin and also very slowly inactivated by E-64. In peptidase family C1 (papain family). Formerly EC 3.4.4.24 and included in EC 3.4.22.4
Links to other databases: BRENDA, EXPASY, KEGG, MEROPS, Metacyc, PDB, CAS registry number: 37189-34-7
References:
1. Brocklehurst, K., Willenbrock, F. and Salih, E. Cysteine proteinases. In New Comprehensive Biochemistry Vol. 16, Hydrolytic Enzymes (Neuberger, A. and Brocklehurst, K., eds), pp. 39-158 (1987) Elsevier, Amsterdam
2. Rowan, A.D., Buttle, D.J. and Barrett, A.J. Ananain: a novel cysteine proteinase found in pineapple stem. Arch. Biochem. Biophys. 267 (1988) 262-270. [PMID: 3196029]
3. Ritonja, A., Rowan, A.B., Buttle, D.J., Rawlings, N.D., Turk, V. and Barrett, A.J. Stem bromelain: amino acid sequence and implications for weak binding to cystatin. FEBS Lett. 247 (1989) 419-429. [PMID: 2714443]
4. Rowan, A.D., Buttle, D.J. and Barrett, A J. The cysteine proteinases of the pineapple plant. Biochem. J. 266 (1990) 869-875. [PMID: 2327970]