Xaa- and -Yaa-Xaa-Gly-XaaGly- (in which Yaa is Met, Ile or Leu, and Xaa is any amino acid)
Reaction: Cleaves proteins of the adenovirus and its host cell at two consensus sites: -Yaa-Xaa-Gly-GlyXaa- and -Yaa-Xaa-Gly-XaaGly- (in which Yaa is Met, Ile or Leu, and Xaa is any amino acid)
Comments: A cysteine endopeptidase from adenoviruses, the type example of peptidase family C5, with a protein fold unlike that known for any other peptidase [2]. Activity is greatly stimulated by the binding to the enzyme of an 11-residue peptide from the adenovirus capsid protein pre-VI at a site separate from the active site [1]
Links to other databases: BRENDA, EXPASY, KEGG, MEROPS, Metacyc, PDB, CAS registry number: 369652-03-9
References
1. Webster, A., Hay, R.T. and Kemp, G. The adenovirus protease is activated by a virus-coded disulphide-linked peptide. Cell 72 (1993) 274-275. [PMID: 8422686]
2. Ding, J.Z., McGrath, W.J., Sweet, R.M. and Mangel, W.F. Crystal structure of the human adenovirus proteinase with its 11 residue cofactor. EMBO J. 15 (1996) 1778-1783. [PMID: 8617222]
3. Weber, J.M. Adenovirus protease. In: Handbook of Proteolytic Enzymes (Barrett, A.J., Rawlings, N.D. and Woessner, J.F. eds), pp. 741-743 (1998) Academic Press, London