Reaction: Broad endopeptidase specificity
Other name(s): calcium-activated neutral protease II; m-calpain; milli-calpain
Comments: Type example of peptidase family C2. Requires Ca2+ at millimolar concentrations for activity. Cytosolic in animal cells. The active enzyme molecule is a heterodimer in which the large subunit contains the peptidase unit, and the small subunit is also a component of EC 3.4.22.52, calpain-1.
Links to other databases: BRENDA, EXPASY, KEGG, MEROPS, Metacyc, PDB, CAS registry number: 702693-80-9
References:
1. Strobl, S., Fernandez-Catalan, C., Braun, M., Huber, R., Masumoto, H., Nakagawa, K., Irie, A., Sorimachi, H., Bourenkow, G., Bartunik, H., Suzuki, K. and Bode, W. The crystal structure of calcium-free human m-calpain suggests an electrostatic switch mechanism for activation by calcium. Proc. Natl. Acad. Sci. USA 97 (2000) 588-592. [PMID: 10639123]
2. Dutt, P., Spriggs, C.N., Davies, P.L., Jia, Z. and Elce, J.S. Origins of the difference in Ca2+ requirement for activation of μ- and m-calpain. Biochem. J. 367 (2002) 263-269. [PMID: 12014988]