Tyr
Reaction: Preferential cleavage in neurotensin: Pro10Tyr
Other names: neurotensin endopeptidase; endopeptidase 24.16; endo-oligopeptidase B (proline-endopeptidase)
Comments: No absolute requirement for a prolyl bond: the enzyme acts on some peptides, such as dynorphin 1-8, that do not contain proline, and does not act on some others that do. In peptidase family M3 (thimet oligopeptidase family)
Links to other databases: BRENDA, EXPASY, KEGG, MEROPS, Metacyc, PDB, CAS registry number: 149371-24-4
References:
1. Checler, F., Vincent, J.P. and Kitabgi, P. Purification and characterization of a novel neurotensin-degrading peptidase from rat brain synaptic membranes. J. Biol. Chem. 261 (1986) 11274-11281. [PMID: 3525564]
2. Barelli, H., Vincent, J.-P. and Checler, F. Peripheral inactivation of neurotensin. Isolation and characterization of a metallopeptidase from rat ileum. Eur. J. Biochem. 175 (1988) 481-489. [PMID: 3409880]
3. Checler, F., Barelli, H. and Vincent, J.-P. Tissue distribution of a novel neurotensin-degrading metallopeptidase. An immunological approach using monospecific polyclonal antibodies. Biochem. J. 257 (1989) 549-554. [PMID: 2649078]