Reaction: Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'
Other names: Astacus proteinase; crayfish small-molecule proteinase
Comments: A 22.6 kDa digestive endopeptidase from the cardia of the crayfish Astacus fluviatilis. Type example of peptidase family M12. Formerly EC 3.4.99.6
Links to other databases: BRENDA, EXPASY, KEGG, MEROPS, Metacyc, PDB, CAS registry number: 143179-21-9
References:
1. Krauhs, E., Dörsam, H., Little, M., Zwilling, R. and Ponstingl, H. A protease from Astacus fluviatilis as an aid in protein sequencing. Anal. Biochem. 119 (1982) 153-157. [PMID: 7041692]
2. Titani, K., Torff, H.-J., Hormel, S., Kumar, S., Walsh, K.A., Rödl, J., Neurath, H. and Zwilling, R. Amino acid sequence of a unique protease from the crayfish Astacus fluviatilis. Biochemistry 26 (1987) 222-226. [PMID: 3548817]
3. Stöcker, W., Wolz, R.L., Zwilling, R., Strydom, D.J. and Auld, D.S. Astacus protease, a zinc metalloenzyme. Biochemistry 27 (1988) 5026-5032
4. Stöcker, W., Ng, M. and Auld, D.S. Fluorescent oligopeptide substrates for kinetic characterization of the specificity of Astacus protease. Biochemistry 29 (1990) 10418-10425. [PMID: 2261483]