Reaction: Similar, but not identical, to that of thermolysin
Other names: Bacillus metalloendopeptidase; Bacillus subtilis neutral proteinase; anilozyme P 10; Bacillus metalloproteinase; Bacillus neutral proteinase; megateriopeptidase
Comments: Variants of this enzyme have been found in species of Bacillus including B. subtilis [1,6], B. amyloliquefaciens [5], B. megaterium (megateriopeptidase, [2]), B. mesentericus [10], B. cereus [3,8,9] and B. stearothermophilus [7]. In peptidase family M4 (thermolysin family). Formerly included in EC 3.4.24.4
Links to other databases: BRENDA, EXPASY, KEGG, MEROPS, Metacyc, PDB, CAS registry number: 9080-56-2
References:
1. Morihara, K., Tsuzuki, H. and Oka, T. Comparison of the specificities of various neutral proteinases from microorganisms. Arch. Biochem. Biophys. 123 (1968) 572-588. [PMID: 4967801]
2. Millet, J. and Acher, R. Spécificité de la mégatériopeptidase: une amino-endopeptidase à caractère hydrophobe. Eur. J. Biochem. 9 (1969) 456-462. [PMID: 4980359]
3. Feder, J., Keay, L., Garrett, L.R., Cirulis, N., Moseley, M.H. and Wildi, B.S. Bacillus cereus neutral protease. Biochim. Biophys. Acta 251 (1971) 74-78. [PMID: 5002444]
4. Holmquist, B. and Vallee, B.L. Esterase activity of zinc neutral proteases. Biochemistry 15 (1976) 101-107. [PMID: 2276]
5. Vasantha, N., Thompson, L.D., Rhodes, C., Banner, C., Nagle, J. and Filpula, D. Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein. J. Bacteriol. 159 (1984) 811-819. [PMID: 6090391]
6. Yang, M.Y., Ferrari, E. and Henner, D.J. Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation. J. Bacteriol. 160 (1984) 15-21. [PMID: 6090407]
7. Takagi, M., Imanaka, T. and Aiba, S. Nucleotide sequence and promoter region for the neutral protease gene from Bacillus stearothermophilus. J. Bacteriol. 163, (1985) 824-831. [PMID: 2993245]
8. Sidler, W., Niederer, E., Suter, F. and Zuber, H. The primary structure of Bacillus cereus neutral proteinase and comparison with thermolysin and Bacillus subtilis neutral proteinase. Biol. Chem. Hoppe-Seyler 367 (1986) 643-657. [PMID: 3092843]
9. Pauptit, R.A., Karlson, R., Picot, D., Jenkins, J.A., Niklaus-Reimer, A.-S. and Jansonius, J.N. Crystal structure of neutral protease from Bacillus cereus refined at 3.0 Å resolution and comparison with the homologous but more thermostable enzyme thermolysin. J. Mol. Biol. 199 (1988) 525-537. [PMID: 3127592]
10. Stoeva, S., Kleinschmidt, T., Mesrob, B. and Braunitzer, G. Primary structure of a zinc protease from Bacillus mesentericus strain 76. Biochemistry 29 (1990) 527-534. [PMID: 2302386]