IUBMB Enzyme Nomenclature


Accepted name: serralysin

Reaction: Preferential cleavage of bonds with hydrophobic residues in P1'

Other names: Pseudomonas aeruginosa alkaline proteinase; Escherichia freundii proteinase; Serratia marcescens extracellular proteinase; Serratia marcescens metalloproteinase; Pseudomonas aeruginosa alk. protease; Serratia marcescens metalloprotease

Comments: A 50 kDa extracellular endopeptidase from Pseudomonas aeruginosa [1,2,6], Escherichia freundii [3], Serratia marcescens [4,5,6] and Erwinia chrysanthemi [7]. There is broad specificity in cleavage of the insulin B chain, with some species variations. The pH optimum for digesting various proteins is about 9 - 10. In peptidase family M10 (interstitial collagenase family). Formerly included in EC

Links to other databases: BRENDA, EXPASY, KEGG, MEROPS, Metacyc, PDB, CAS registry number: 70851-98-8


1. Morihara, K., Tsuzuki, H. and Oka, T. Comparison of the specificities of various neutral proteinases from microorganisms. Arch. Biochem. Biophys. 123 (1968) 572-588. [PMID: 4967801]

2. Morihara, K., Tsuzuki, H. and Oka, T. On the specificity of Pseudomonas aeruginosa alkaline proteinase with synthetic peptides. Biochim. Biophys. Acta 309 (1973) 414-429. [PMID: 4199986]

3. Nakajima, M., Mizusawa, K. and Yoshida, F. Purification and properties of an extracellular proteinase of psychrophilic Escherichia freundii. Eur. J. Biochem.44 (1974) 87-96. [PMID: 4212288]

4. Decedue, C.J., Broussard, E.A., II, Larson, A.D. and Braymer, H.D. Purification and characterization of the extracellular proteinase of Serratia marcescens. Biochim. Biophys. Acta 569 (1979) 293-301. [PMID: 383155]

5. Doerr, M. and Traub, W.H. Purification and characterization of two Serratia marcescens proteases. Zentralbl. Bakteriol., Mikrobiol. Hyg. Ser. A 257 (1984) 6-19. [PMID: 6380155]

6. Nakahama, K., Yoshimura, K., Marumoto, R., Kikuchi, M., Lee, I.S., Hase, T. and Matsubara, H. Cloning and sequencing of Serratia protease gene. Nucleic Acids Res. 14 (1986) 5843-5856. [PMID: 3016665]

7. Dahler, G.S., Barras, F. and Keen, N.T. Cloning of genes encoding extracellular metalloproteases from Erwinia chrysanthemi EC16. J. Bacteriol. 172 (1990) 5803-5815. [PMID: 2211513]

8. Okuda, K., Morihara, K., Atsumi, Y., Takeuchi, H., Kawamoto, S., Kawasaki, H., Suzuki, K. and Fukushima, J. Complete nucleotide sequence of the structural gene for alkaline proteinase from Pseudomonas aeruginosa IFO 3455. Infect. Immun. 58 (1990) 4083-4088. [PMID: 2123832]

[EC created 1972 as EC, part transferred 1992 to EC]

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